| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Whitaker, Gabriel L (18)
|
INCSC |
58.80 |
57.23 |
-1.57 |
| 2 |
Scott, John J (18)
|
OHUSA |
|
57.29 |
- |
| 3 |
Prolo, Bradley R (16)
|
CASCAT |
58.60 |
57.57 |
-1.03 |
| 4 |
DeMercurio, Jacob C (17)
|
NTTFA |
58.18 |
57.69 |
-0.49 |
| 5 |
Jhong, Christopher A (15)
|
PCPLS |
59.82 |
57.94 |
-1.88 |
| 6 |
Lutzker, Aaron N (18)
|
PCWCAB |
58.79 |
58.11 |
-0.68 |
| 7 |
Perera, Nicholas V (15)
|
OHCM |
1:00.08 |
58.13 |
-1.95 |
| 8 |
Mitchell, John P (17)
|
GAMAAC |
58.06 |
58.18 |
0.12 |
| 9 |
Hofstadter, William A (18)
|
CAJCCW |
59.60 |
58.50 |
-1.10 |
| 10 |
Colin, John L (17)
|
IADASH |
59.97 |
58.55 |
-1.42 |
| 11 |
Poppe, Hank H (16)
|
PCWCAB |
1:00.51 |
58.68 |
-1.83 |
| 12 |
Hrosik, Ryan M (15)
|
INMAKO |
58.84 |
58.78 |
-0.06 |
| 13 |
Graber, Daniel J (18)
|
COFST |
1:01.32 |
58.86 |
-2.46 |
| 14 |
Toth, Viktor (18)
|
FLDBS |
57.96 |
58.99 |
1.03 |
| 15 |
Curtis, Dylan P (18)
|
OHUN-0 |
58.92 |
59.09 |
0.17 |
| 16 |
Russiello, Ian D (18)
|
PVFISH |
58.36 |
59.46 |
1.10 |
| 17 |
Kirkpatrick, Calvin N (18)
|
PCWCAB |
59.60 |
59.72 |
0.12 |
| 18 |
Schlemovitz, Aaron J (18)
|
NJSHY |
1:01.87 |
59.77 |
-2.10 |
| 19 |
Courtney, James V (16)
|
SECMSA |
1:00.45 |
59.93 |
-0.52 |
| 20 |
Holmes, Matthew D (18)
|
FLCBS |
1:04.62 |
1:00.17 |
-4.45 |
| 21 |
Andrew, Michael C (16)
|
MVIS |
53.46 |
1:01.34 |
7.88 |